Calpain S1 antibody [AT1D11]

Cat. No. GTX57626

Host	Mouse
Clonality	Monoclonal
Clone Name	AT1D11
Isotype	IgG1
Application	WB, ICC/IF, FACS
Reactivity	Human, Mouse
Package	100 μl ($399)

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Datasheet
Document

View product citations for antibody GTX57626 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
FACS	Assay dependent

Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT1D11 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CAPNS3 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calpain small subunit 1 , CALPAIN4 , CANP , CANPS , CAPN4 , CAPNS1 , CDPS , CSS1 , Calpain S1

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene is a member of the calpain small subunit family. Calpains are calcium-dependent cysteine proteinases that are widely distributed in mammalian cells. Calpains operate as heterodimers, comprising a specific large catalytic subunit (calpain 1 subunit in Calpain I, and calpain 2 subunit in Calpain II), and a common small regulatory subunit encoded by this gene. This encoded protein is essential for the stability and function of both calpain heterodimers, whose proteolytic activities influence various cellular functions including apoptosis, proliferation, migration, adhesion, and autophagy. Calpains have been implicated in neurodegenerative processes, such as myotonic dystrophy. A pseudogene of this gene has been defined on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Database

Gene ID: 826 CAPNS1
UniProt: P04632 CAPNS1

Research Area

DATA IMAGES

GTX57626 FACS Image

FACS analysis of HeLa cells using GTX57626 Calpain S1 antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg

GTX57626 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57626 Calpain S1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

GTX57626 WB Image

WB analysis of various samples using GTX57626 Calpain S1 antibody.
Lane 1 : Recombinant Human CAPNS1 protein (50 ng)
Lane 2 : HeLa whole cell lysate (40 μg)
Lane 3 : A431 whole cell lysate (40 μg)
Lane 4 : 293T whole cell lysate (40 μg)
Lane 5 : Balb/3T3 whole cell lysate (40 μg)
Lane 6 : U87-MG whole cell lysate (40 μg)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty