Casein Kinase 2 alpha antibody

Name: Casein Kinase 2 alpha antibody
Brand: GeneTex
SKU: GTX107576
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Casein Kinase 2 alpha antibody used in Western Blot (WB). GTX107576

Anti-Casein Kinase 2 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX107576

Cat. No. GTX107576

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

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Datasheet
Document

View product citations for antibody GTX107576 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

A549 , JC , BCL-1

Predict Reactivity

Rat, Zebrafish, Rabbit, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Casein Kinase 2 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10616991

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CK2A1 antibody , CKII antibody , CSNK2A1 antibody , casein kinase II alpha 1 subunit antibody , CK II alpha antibody , protein kinase CK2 antibody , CK2 catalytic subunit alpha antibody , casein kinase II subunit alpha antibody , "casein kinase 2 , alpha 1 polypeptide antibody"

Background

Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene. [provided by RefSeq]

Research Area

DATA IMAGES

GTX107576 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 alpha antibody (GTX107576) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX107576 WB Image

Sample (30 ug of whole cell lysate)
B: JC
C: BCL-1
10% SDS PAGE
GTX107576 diluted at 1:1000

GTX107576 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using Casein Kinase 2 alpha 1(GTX107576) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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REVIEW