Casein Kinase 2 alpha antibody

Name: Casein Kinase 2 alpha antibody
Brand: GeneTex
SKU: GTX107897
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Casein Kinase 2 alpha antibody used in Western Blot (WB). GTX107897

Anti-Casein Kinase 2 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX107897

Anti-Casein Kinase 2 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107897

Cat. No. GTX107897

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Casein Kinase 2 alpha products

View product citations for antibody GTX107897 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

mouse brain , 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , PC-12 , Rat2 , rat brain

Predict Reactivity

Zebrafish, Rabbit, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Casein Kinase 2 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950048

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CK2A1 antibody , CKII antibody , CSNK2A1 antibody , casein kinase II alpha 1 subunit antibody , CK II alpha antibody , protein kinase CK2 antibody , CK2 catalytic subunit alpha antibody , casein kinase II subunit alpha antibody , casein kinase 2 , alpha 1 polypeptide antibody

Background

Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene. [provided by RefSeq]

Research Area

DATA IMAGES

GTX107897 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 alpha antibody (GTX107897) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX107897 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 alpha antibody (GTX107897) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107897 WB Image

Casein Kinase 2 alpha antibody detects CSNK2A1 protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
10% SDS-PAGE
Casein Kinase 2 alpha antibody (GTX107897) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107897 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Casein Kinase 2 alpha 1(GTX107897) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107897 ICC/IF Image

Casein Kinase 2 alpha antibody detects CSNK2A1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CSNK2A1 protein stained by Casein Kinase 2 alpha antibody (GTX107897) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX107897 WB Image

The data was published in the journal Oncogene in 2017. PMID: 28436950

REFERENCE

Application Reference

Gong L et al. Nat Commun 2022; 13 (1) : 6781 CK2-mediated phosphorylation of SUZ12 promotes PRC2 function by stabilizing enzyme active site.

Lan YC et al. Int J Med Sci 2020; 17 (1) : 13-20 Effects of Casein Kinase 2 Alpha 1 Gene Expression on Mice Liver Susceptible to Type 2 Diabetes Mellitus and Obesity.

Jin X et al. Mol Cell Biol 2018; (Epub) modulation of hsf1 activity through silencing of ser303/ser307 phosphorylation supports a metabolic program leading to age-related obesity and * resistance.

Application : WB

Reactivity : Mouse

von Morgen P et al. Oncogene 2017; 36 (34) : 4943-4950 MRE11 stability is regulated by CK2-dependent interaction with R2TP complex.

Jin X et al. J Biol Chem 2017; (Epub) Abrogation of heat shock factor 1 (Hsf1) phosphorylation deregulates its activity and lowers activation threshold, leading to obesity in mice.

Application : WB

Reactivity : Mouse

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