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Caspase 3 (cleaved Asp175) antibody

Anti-Caspase 3 (cleaved Asp175) antibody used in IHC (Paraffin sections) (IHC-P). GTX86900
Anti-Caspase 3 (cleaved Asp175) antibody used in Western Blot (WB). GTX86900

Cat. No. GTX86900

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all Caspase 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Caspase 3 (157-206).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CASP3 , CPP32 , CPP32B , SCA1 , caspase 3 , Caspase 3

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Caspase 3 (cleaved Asp175) antibody used in IHC (Paraffin sections) (IHC-P). GTX86900

GTX86900 IHC-P Image

IHC-P analysis of human breast tissue using GTX86900 Caspase 3 (cleaved Asp175) antibody.

Anti-Caspase 3 (cleaved Asp175) antibody used in Western Blot (WB). GTX86900

GTX86900 WB Image

WB analysis of HeLa cells treated with Etoposide 25uM (60mins) lysate using GTX86900 Caspase 3 (cleaved Asp175) antibody. The lane on the right is blocked with the synthesized peptide.

REFERENCE

Application Reference

Meng-Hui Zhang et al. Bioengineered 2022; 13 (4) : 10047-10060 Cleavage stimulation factor 2 promotes malignant progression of liver hepatocellular carcinoma by activating phosphatidylinositol 3'-kinase/protein kinase B/mammalian target of rapamycin pathway.
Application : WB
Zhao X et al. Cancer Cell Int 2021; 21 (1) : 644 Silencing of the lncRNA H19 enhances sensitivity to X-ray and carbon-ions through the miR-130a-3p /WNK3 signaling axis in NSCLC cells.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399