Caspase 8 antibody [2G12]

Cat. No. GTX34167

Host	Mouse
Clonality	Monoclonal
Clone Name	2G12
Isotype	IgG1
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399)

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Datasheet
Document

View product citations for antibody GTX34167 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:2000
IHC-P	1:200-1:500

Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Protein

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ALPS2B,CAP4,CASP8,Casp8,FLICE,MACH,MCH5,caspase 8,Caspase 8

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]

Database

Gene ID: 841 CASP8
UniProt: Q14790 CASP8

Research Area

DATA IMAGES

GTX34167 IHC-P Image

IHC-P analysis of human colon tissue using GTX34167 Caspase 8 antibody [2G12]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34167 IHC-P Image

IHC-P analysis of human stomach cancer tissue using GTX34167 Caspase 8 antibody [2G12]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34167 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX34167 Caspase 8 antibody [2G12]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34167 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34167 Caspase 8 antibody [2G12]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34167 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX34167 Caspase 8 antibody [2G12]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34167 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX34167 Caspase 8 antibody [2G12].

GTX34167 WB Image

WB analysis of various sample lysates using GTX34167 Caspase 8 antibody [2G12].
Lane 1 : HeLa cell lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat brain tissue lysate

GTX34167 IHC-P Image

IHC-P analysis of human liver tissue using GTX34167 Caspase 8 antibody [2G12]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34167 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX34167 Caspase 8 antibody [2G12]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty

Caspase 8 antibody [2G12]

Cat. No. GTX34167

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX34167 IHC-P Image

GTX34167 IHC-P Image

GTX34167 IHC-P Image

GTX34167 IHC-P Image

GTX34167 IHC-P Image

GTX34167 IHC-P Image

GTX34167 WB Image

GTX34167 IHC-P Image

GTX34167 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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