APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Jurakt , Jurkat (10 μM Etoposide treatment for 24 hr)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
2.58 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Caspase 8 p10. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2887315
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
caspase 8 , ALPS2B , CAP4 , Casp-8 , FLICE , MACH , MCH5
Cellular Localization
Cytoplasm
Background
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX134589 WB Image
Untreated (–) and treated (+) Jurkat whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 8 p10 antibody (GTX134589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX134589 IHC-P Image
Caspase 8 p10 antibody detects Caspase 8 p10 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Caspase 8 p10 stained by Caspase 8 p10 antibody (GTX134589) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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REVIEW
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