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Caspase 9 (cleaved Asp315) antibody

Anti-Caspase 9 (cleaved Asp315) antibody used in IHC (Paraffin sections) (IHC-P). GTX86945
Anti-Caspase 9 (cleaved Asp315) antibody used in Western Blot (WB). GTX86945

Cat. No. GTX86945

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all Caspase 9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Product Note

Caspase 9 (cleaved Asp315) antibody detects endogenous levels of fragment of activated Caspase 9 resulting from cleavage adjacent to Asp315.

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Caspase 9 (266-315).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

APAF3 , CASP9 , ICELAP6 , MCH6 , PPP1R56 , caspase 9 , Caspase 9

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Database

Research Area

DATA IMAGES

Anti-Caspase 9 (cleaved Asp315) antibody used in IHC (Paraffin sections) (IHC-P). GTX86945

GTX86945 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX86945 Caspase 9 (cleaved Asp315) antibody. The picture on the right is blocked with the synthesized peptide.

Anti-Caspase 9 (cleaved Asp315) antibody used in Western Blot (WB). GTX86945

GTX86945 WB Image

WB analysis of 293 cell lysates using GTX86945 Caspase 9 (cleaved Asp315) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399