APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
DDDDK-tagged CTSA-transfected 293T , U87-MG
Predict Reactivity
Rat, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.29 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Cathepsin A. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886806
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cathepsin A , GLB2 , GSL , NGBE , PPCA , PPGB
Cellular Localization
Lysosome
Background
This gene encodes a member of the peptidase S10 family of serine carboxypeptidases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate two chains that comprise the heterodimeric active enzyme. This enzyme possesses deamidase, esterase and carboxypeptidase activities and acts as a scaffold in the lysosomal multienzyme complex. Mutations in this gene are associated with galactosialidosis. [provided by RefSeq, Nov 2015]
Database
DATA IMAGES
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GTX133016 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cathepsin A antibody (GTX133016) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX133016 IHC-P Image
Cathepsin A antibody detects Cathepsin A protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.Cathepsin A stained by Cathepsin A antibody (GTX133016) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133016 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cathepsin A antibody (GTX133016) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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REVIEW
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