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Cathepsin D antibody

Anti-Cathepsin D antibody used in Western Blot (WB). GTX133019
Anti-Cathepsin D antibody used in Western Blot (WB). GTX133019
Anti-Cathepsin D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133019

Cat. No. GTX133019

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Cathepsin D products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

MCF-7 , MDA-MB-231 , Raw264.7

Predict Reactivity

Rat, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cathepsin D. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886807

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cathepsin D , CLN10 , CPSD , HEL-S-130P

Cellular Localization

Lysosome , Melanosome

Background

This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cathepsin D antibody used in Western Blot (WB). GTX133019

GTX133019 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cathepsin D antibody (GTX133019) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cathepsin D antibody used in Western Blot (WB). GTX133019

GTX133019 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cathepsin D antibody (GTX133019) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cathepsin D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133019

GTX133019 ICC/IF Image

Cathepsin D antibody detects Cathepsin D protein at lysosome by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: Cathepsin D stained by Cathepsin D antibody (GTX133019) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Audrey Voisin et al. Oxidative Medicine and Cellular Longevity 2019; 2019 : Article ID 5637075 Cathepsin B pH-Dependent Activity Is Involved in Lysosomal Dysregulation in Atrophic Age-Related Macular Degeneration.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169