APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Recommended starting dilution is 1:500. |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
Based on internal testing result, this antibody does not cross react with human CTSB, human CTSD, human CTSE, human CTSF, human CTSH, human CTSK, human CTSL, human CTSW, and human CTSZ.
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT1F9 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CTSS protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cathepsin S , CTSS , Cathepsin S
Cellular Localization
Lysosome
Background
The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]
Database
Research Area
DATA IMAGES
![Anti-Cathepsin S antibody [AT1F9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57592](/templates/default/js/lightbox/images/loading.gif "Anti-Cathepsin S antibody [AT1F9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57592")
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GTX57592 ICC/IF Image
ICC/IF analysis of A549 cells using GTX57592 Cathepsin S antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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GTX57592 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57592 Cathepsin S antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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GTX57592 WB Image
WB analysis of various samples using GTX57592 Cathepsin S antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : U87-MG whole cell lysate
Lane 3 : A549 whole cell lysate
Loading : 40 μg
Dilution : 1:500
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GTX57592 WB Image
WB analysis of various samples using GTX57592 Cathepsin S antibody.
Lane 1 : Recombinant Human CTSB protein
Lane 2 : Recombinant Human CTSD protein
Lane 3 : Recombinant Human CTSE protein
Lane 4 : Recombinant Human CTSF protein
Lane 5 : Recombinant Human CTSH protein
Lane 6 : Recombinant Human CTSK protein
Lane 7 : Recombinant Human CTSL protein
Lane 8 : Recombinant Human CTSS protein
Lane 9 : Recombinant Human CTSW protein
Lane 10 : Recombinant Human CTSZ protein
Loading : 20 ng
Dilution : 1:1000
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REFERENCE
There are currently no references for Cathepsin S antibody [AT1F9] (GTX57592). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cathepsin S antibody [AT1F9] (GTX57592). Be the first to share your experience with this product.
![Anti-Cathepsin S antibody [AT1F9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57592](/upload/website/prouct_img/small/GTX57592/GTX57592_20200204_IF3_w_23061123_384.webp "Anti-Cathepsin S antibody [AT1F9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57592")
![Anti-Cathepsin S antibody [AT1F9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57592](/upload/website/prouct_img/small/GTX57592/GTX57592_20200204_IF2_w_23061123_388.webp "Anti-Cathepsin S antibody [AT1F9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57592")
![Anti-Cathepsin S antibody [AT1F9] used in Western Blot (WB). GTX57592](/upload/website/prouct_img/small/GTX57592/GTX57592_20200204_WB2_w_23061123_447.webp "Anti-Cathepsin S antibody [AT1F9] used in Western Blot (WB). GTX57592")
![Anti-Cathepsin S antibody [AT1F9] used in Western Blot (WB). GTX57592](/upload/website/prouct_img/small/GTX57592/GTX57592_20200204_WB1_w_23061123_770.webp "Anti-Cathepsin S antibody [AT1F9] used in Western Blot (WB). GTX57592")
![Anti-Cathepsin S antibody [AT1F9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57592](/upload/website/prouct_img/normal/GTX57592/GTX57592_20200204_IF3_w_23061123_384.webp "Anti-Cathepsin S antibody [AT1F9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57592")
![Anti-Cathepsin S antibody [AT1F9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57592](/upload/website/prouct_img/normal/GTX57592/GTX57592_20200204_IF2_w_23061123_388.webp "Anti-Cathepsin S antibody [AT1F9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57592")
![Anti-Cathepsin S antibody [AT1F9] used in Western Blot (WB). GTX57592](/upload/website/prouct_img/normal/GTX57592/GTX57592_20200204_WB2_w_23061123_447.webp "Anti-Cathepsin S antibody [AT1F9] used in Western Blot (WB). GTX57592")
![Anti-Cathepsin S antibody [AT1F9] used in Western Blot (WB). GTX57592](/upload/website/prouct_img/normal/GTX57592/GTX57592_20200204_WB1_w_23061123_770.webp "Anti-Cathepsin S antibody [AT1F9] used in Western Blot (WB). GTX57592")