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Caveolin 1 (phospho Tyr14) antibody

Orthogonal
Anti-Caveolin 1 (phospho Tyr14) antibody used in Western Blot (WB). GTX79117

Cat. No. GTX79117

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Mouse
Package
100 μg ($399)
See all Caveolin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Predict Reactivity

Human, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human Caveolin-1 around the phosphorylation site of tyrosine 14 (H-L-Yp-T-V).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11171193

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BSCL3,CAV1,CGL3,LCCNS,MSTP085,PPH3,VIP21,caveolin 1,Caveolin 1

Cellular Localization

Golgi apparatus membrane,Cell membrane,Membrane,Caveola,Membrane raft

Background

The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.[provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-Caveolin 1 (phospho Tyr14) antibody used in Western Blot (WB). GTX79117

GTX79117 WB Image

Orthogonal

WB analysis of NIH/3T3 cells untreated or treated with H2O2 lysates using GTX79117 Caveolin 1 (phospho Tyr14) antibody (Lane 3 and 4) and Caveolin 1 antibody (Lane 1 and 2).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399