APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
3xFlag-human CETN2-transfected 293T , 293T , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS
Predict Reactivity
Mouse, Sheep, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Centrin 2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886074
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
centrin 2 , CALT , CEN2
Cellular Localization
Centrosome , centriole
Background
Caltractin belongs to a family of calcium-binding proteins and is a structural component of the centrosome. The high level of conservation from algae to humans and its association with the centrosome suggested that caltractin plays a fundamental role in the structure and function of the microtubule-organizing center, possibly required for the proper duplication and segregation of the centrosome. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX129709 WB Image
Centrin 2 antibody detects Centrin 2 protein by western blot analysis. Non-transfected (-) and CETN2-transfected (+, including Flag-tag) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Centrin 2 antibody (GTX129709) diluted by 1:20000.
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GTX129709 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Centrin 2 antibody (GTX129709) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX129709 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Centrin 2 antibody (GTX129709) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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REVIEW
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