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Chikungunya virus nsP2 antibody

Anti-Chikungunya virus nsP2 antibody used in Western Blot (WB). GTX135188
Anti-Chikungunya virus nsP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135188
Anti-Chikungunya virus nsP2 antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135188

Cat. No. GTX135188

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P (cell pellet)

Reactivity

 Chikungunya Virus
Package
100 μl ($399),
25 μl ($169)
See all Chikungunya virus nsP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:50000
ICC/IF Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Chikungunya Virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Chikungunya virus nsP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2909831

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Chikungunya virus nsP2 antibody used in Western Blot (WB). GTX135188

GTX135188 WB Image

Chikungunya viral lysate (0.5 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Chikungunya virus nsP2 antibody (GTX135188) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Chikungunya virus nsP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135188

GTX135188 ICC/IF Image

Chikungunya virus nsP2 antibody detects Chikungunya virus nsP2 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Chikungunya virus nsP2 stained by Chikungunya virus nsP2 antibody (GTX135188) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-Chikungunya virus nsP2 antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135188

GTX135188 IHC-P (cell pellet) Image

Chikungunya virus nsP2 antibody detects Chikungunya virus nsP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mock and Chikungunya virus nsP2 - transfected 293T cell pellet.
Green: Chikungunya virus nsP2 stained by Chikungunya virus nsP2 antibody (GTX135188) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169