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Claspin antibody

Anti-Claspin antibody used in Immunoprecipitation (IP). GTX23720
Anti-Claspin antibody used in Immunoprecipitation (IP). GTX23720
Anti-Claspin antibody used in Proximity ligation assay (PLA). GTX23720
Anti-Claspin antibody used in Western Blot (WB). GTX23720
Anti-Claspin antibody used in IHC (Paraffin sections) (IHC-P). GTX23720

Cat. No. GTX23720

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, PLA

Reactivity

 Human
Package
50 μl ($399)
See all Claspin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2500
IHC-P 1:500 - 1:2000
IP 1 - 5 μg/mg lysate
PLA 1:1000 - 1:10000
Not tested in other applications.

Calculated MW

151 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tris-Citrate/Phosphate

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen recognized by this antibody maps to a region between residues 450 and 500 of human Claspin using the numbering given in entry AAG24515.1 (GeneID 63967).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_369621

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

claspin

Cellular Localization

Nucleus

Background

The product of this gene is an essential upstream regulator of checkpoint kinase 1 and triggers a checkpoint arrest of the cell cycle in response to replicative stress or DNA damage. The protein is also required for efficient DNA replication during a normal S phase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Claspin antibody used in Immunoprecipitation (IP). GTX23720

GTX23720 IP Image

(A)WB analysis of HeLa cell lysate using GTX23720 Claspin antibody.
(B)IP analysis of HeLa cell lysate using GTX23720 Claspin antibody.

Anti-Claspin antibody used in Immunoprecipitation (IP). GTX23720

GTX23720 IP Image

IP analysis of HeLa cell lysate using GTX23720 Claspin antibody.
IP reaction : 6μg antibody / 1.0 mg lysate
Dilution : 1 μg/ml
Loading: 20% of IP reaction

Anti-Claspin antibody used in Proximity ligation assay (PLA). GTX23720

GTX23720 PLA Image

PLA analysis of HeLa cells using GTX23720 Claspin antibody and anti-human CHK1 antibody.
Green : Actin
Red : positive signal
Blue : DAPI

Anti-Claspin antibody used in Western Blot (WB). GTX23720

GTX23720 WB Image

WB analysis of HeLa cell lysate using GTX23720 Claspin antibody.
Dilution : 1 μg/ml
Loading : 50μg

Anti-Claspin antibody used in IHC (Paraffin sections) (IHC-P). GTX23720

GTX23720 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX23720 Claspin antibody.
Dilution : 1 μg/ml

REFERENCE

Application Reference

Liu Y et al. J Biol Chem 2010; 285 (8) : 5974-82 Interactions of human mismatch repair proteins MutSalpha and MutLalpha with proteins of the ATR-Chk1 pathway.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399