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Claudin 10 antibody

Anti-Claudin 10 antibody used in Western Blot (WB). GTX01076
Anti-Claudin 10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01076
Anti-Claudin 10 antibody used in IHC (Paraffin sections) (IHC-P). GTX01076

Cat. No. GTX01076

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Claudin 10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Chicken, Pig, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Claudin 10, corresponding to a region within C-terminal amino acids.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

claudin 10 , CLDN10 , CPETRL3 , OSPL , Claudin 10

Cellular Localization

Cell junction,Tight junction,Cell membrane

Background

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The expression level of this gene is associated with recurrence of primary hepatocellular carcinoma. Six alternatively spliced transcript variants encoding different isoforms have been reported, but the transcript sequences of some variants are not determined.[provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-Claudin 10 antibody used in Western Blot (WB). GTX01076

GTX01076 WB Image

WB analysis of 293 cell lysate using GTX01076 Claudin 10 antibody.

Anti-Claudin 10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01076

GTX01076 ICC/IF Image

ICC/IF analysis of PFA-fixed LOVO cells using GTX01076 Claudin 10 antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-Claudin 10 antibody used in IHC (Paraffin sections) (IHC-P). GTX01076

GTX01076 IHC-P Image

IHC-P analysis of mouse kidney tissue section using GTX01076 Claudin 10 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399