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Claudin 5 antibody

Anti-Claudin 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX49370
Anti-Claudin 5 antibody used in Western Blot (WB). GTX49370

Cat. No. GTX49370

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399)
See all Claudin 5 products

APPLICATION

Application Note

WB: 1:500-1:1000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids 171-220 of Human Claudin-5.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

RRID

AB_2811720

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

claudin 5 , AWAL , BEC1 , CPETRL1 , TMDVCF , TMVCF

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2018]

Database

Research Area

DATA IMAGES

Anti-Claudin 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX49370

GTX49370 IHC-P Image

Immunohistochemistry (IHC) analyzes of Claudin-5 pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Anti-Claudin 5 antibody used in Western Blot (WB). GTX49370

GTX49370 WB Image

Western blot (WB) analysis of Claudin-5 pAb at 1:500 dilution Lane1:Hela cell lysate treated with LPS Lane2:Mouse liver tissue lysate Lane3:Rat lung tissue lysate

REFERENCE

Application Reference

Shen S et al. Bioengineered 2022; 13 (3) : 7209-7220 The protective effects of butorphanol tartrate against homocysteine-induced blood-brain barrier dysfunction.
Application : IHC-P
Reactivity : Mouse
Watanabe D et al. Pharmaceutics 2021; 13 (9) Characterization of a Primate Blood-Brain Barrier Co-Culture Model Prepared from Primary Brain Endothelial Cells, Pericytes and Astrocytes.
Application : WB
Reactivity : Monkey
Wang M et al. Aging (Albany NY) 2020; 12 (16) : 16099-16110 IL-21 mediates microRNA-423-5p /claudin-5 signal pathway and intestinal barrier function in inflammatory bowel disease.
Application : WB
Reactivity : Human
Chen P et al. Med Sci Monit 2020; 26 : e922009 Basic Fibroblast Growth Factor (bFGF) Protects the Blood-Brain Barrier by Binding of FGFR1 and Activating the ERK Signaling Pathway After Intra-Abdominal Hypertension and Traumatic Brain Injury.
Application : IHC-P
Reactivity : Rat
Chen P et al. Med Sci Monit 2019; 25 : 7191-7201 Basic Fibroblast Growth Factor Reduces Permeability and Apoptosis of Human Brain Microvascular Endothelial Cells in Response to Oxygen and Glucose Deprivation Followed by Reoxygenation via the Fibroblast Growth Factor Receptor 1 (FGFR1)/ERK Pathway.
Application : WB
Reactivity : Human
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Application Reference
Shen S et al. Bioengineered 2022; 13 (3):7209-7220 The protective effects of butorphanol tartrate against homocysteine-induced blood-brain barrier dysfunction.
Application : IHC-P
Reactivity : Mouse
Watanabe D et al. Pharmaceutics 2021; 13 (9) Characterization of a Primate Blood-Brain Barrier Co-Culture Model Prepared from Primary Brain Endothelial Cells, Pericytes and Astrocytes.
Application : WB
Reactivity : Monkey
Wang M et al. Aging (Albany NY) 2020; 12 (16):16099-16110 IL-21 mediates microRNA-423-5p /claudin-5 signal pathway and intestinal barrier function in inflammatory bowel disease.
Application : WB
Reactivity : Human
Chen P et al. Med Sci Monit 2020; 26:e922009 Basic Fibroblast Growth Factor (bFGF) Protects the Blood-Brain Barrier by Binding of FGFR1 and Activating the ERK Signaling Pathway After Intra-Abdominal Hypertension and Traumatic Brain Injury.
Application : IHC-P
Reactivity : Rat
Chen P et al. Med Sci Monit 2019; 25:7191-7201 Basic Fibroblast Growth Factor Reduces Permeability and Apoptosis of Human Brain Microvascular Endothelial Cells in Response to Oxygen and Glucose Deprivation Followed by Reoxygenation via the Fibroblast Growth Factor Receptor 1 (FGFR1)/ERK Pathway.
Application : WB
Reactivity : Human
Divine C. Nwafor et al. bioRxiv 2019; (Epub) Loss of tissue non-specific alkaline phosphatase (TNAP) enzyme activity in cerebral microvessels is coupled to persistent neuroinflammation and behavioral deficits in late sepsis.
Application : IHC-P
Reactivity : Mouse
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399