APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| 10μg/ml |
| 10μg/ml |
| Assay dependent |
|
Note :
ICC/IF
Strong expression of the protein seen in the nucleus of U2OS cells.
IHC-P
Human Testis shows granular cytoplasmic staining of Leydig cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89642-PEP).
Predict Reactivity
Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence ELETRMDQVTCAA, from the C Terminus of the protein sequence according to NP_543026.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
serine/threonine kinase 35 , CLIK1 , STK35L1
Cellular Localization
Nucleus , Cytoplasm , Nucleolus
Background
The protein encoded by this gene is a kinase that is predominantly found in the nucleus. However, it can interact with PDLIM1/CLP-36 in the cytoplasm and localize to actin stress fibers. The encoded protein may be a regulator of actin stress fibers in nonmuscle cells. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX89642 IHC-P Image
IHC-P analysis of human testis using GTX89642 Clik1 / STK35 antibody, C-term.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 10μg/ml
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GTX89642 FACS Image
FACS analysis of PFA fixed HEK293 cells using GTX89642 Clik1 / STK35 antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml
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GTX89642 WB Image
WB analysis of HEK293 nuclear (A) and human breast cancer (B) lysate using GTX89642 Clik1 / STK35 antibody, C-term.
Dilution : 2μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89642 ICC/IF Image
ICC/IF analysis of PFA fixed U2OS cells using GTX89642 Clik1 / STK35 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
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GTX89642 WB Image
WB analysis of mouse (A) and pig (B) testes lysate using GTX89642 Clik1 / STK35 antibody, C-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for Clik1 / STK35 antibody, C-term (GTX89642). Be the first to share your publications with this product.
REVIEW
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