APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:2000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT1C1 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Cofilin 3 protein.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cofilin 1 , CFL , CFL1 , HELS15 , cofilin , Cofilin 1
Cellular Localization
Nucleus matrix,Cytoplasm,Cytoskeleton,Cell projection,Ruffle membrane,Lamellipodium membrane
Background
The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]
Database
Research Area
DATA IMAGES
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GTX57545 FACS Image
FACS analysis of HeLa cells using GTX57545 Cofilin 1 antibody. Cell Number: 1 x 10⁶ cells Red: Primary antibody Antibody amount: 2-5 μg
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GTX57545 WB Image
WB analysis of HeLa whole cell lysate using GTX57545 Cofilin 1 antibody. Loading : 20 μg Dilution : 1:1000
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GTX57545 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57545 Cofilin 1 antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100
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REFERENCE
There are currently no references for Cofilin 1 antibody [AT1C1] (GTX57545). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cofilin 1 antibody [AT1C1] (GTX57545). Be the first to share your experience with this product.