Collagen II antibody [N1], N-term

Name: Collagen II antibody [N1], N-term
Brand: GeneTex
SKU: GTX100829
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Collagen II antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100829

Anti-Collagen II antibody [N1], N-term used in Western Blot (WB). GTX100829

Cat. No. GTX100829

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Collagen II products

View product citations for antibody GTX100829 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

142 kDa. ( Note )

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Collagen II. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166310

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type II alpha 1 chain , ANFH , AOM , COL11A3 , SEDC , STL1

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq]

Database

Gene ID: 1280 COL2A1
UniProt: P02458 COL2A1

Research Area

DATA IMAGES

GTX100829 IHC-P Image

Collagen II antibody [N1], N-term detects Collagen II protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Collagen II antibody [N1], N-term (GTX100829) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100829 WB Image

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen II antibody [N1], N-term (GTX100829) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100829 IHC-P Image

Collagen II antibody [N1], N-term detects Collagen II protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Collagen II antibody [N1], N-term (GTX100829) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Chang YH et al. Stem Cells Int 2021; 2021 : 2989054 Human Umbilical Mesenchymal Stromal Cells Mixed with Hyaluronan Transplantation Decreased Cartilage Destruction in a Rabbit Osteoarthritis Model.

Application : IHC-P

Muhammad SA et al. Cartilage 2019; : 1947603519876333 Optimization of Protocol for Isolation of Chondrocytes from Human Articular Cartilage.

Application : ICC/IF

Ding DC et al. Int J Biol Sci 2018; 14 (11) : 1586-1598 Dedifferentiation of human uterine polyp stem cells into embryo-like cells during inducing pluripotency.

Application : IHC-P

Reactivity : Human

Chang YH et al. Tzu Chi Medical Journal 2018; (Epub) Human umbilical cord-derived mesenchymal stem cells reduce monosodium iodoacetate-induced apoptosis in cartilage.

Application : IHC-P

Reactivity : Human

Qu J et al. Clin Exp Pharmacol Physiol 2015; (Epub) PFKFB3 modulates glycolytic metabolism and alleviates endoplasmic reticulum stress in human osteoarthritis cartilage.

Application : WB

Reactivity : Human

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