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Collagen VI alpha 2 antibody

Anti-Collagen VI alpha 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55141
Anti-Collagen VI alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX55141
Anti-Collagen VI alpha 2 antibody used in Western Blot (WB). GTX55141

Cat. No. GTX55141

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

109 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of Collagen VI alpha 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type VI alpha 2 chain , BTHLM1 , PP3610 , UCMD1

Cellular Localization

Membrane

Background

This gene encodes one of the three alpha chains of type VI collagen, a beaded filament collagen found in most connective tissues. The product of this gene contains several domains similar to von Willebrand Factor type A domains. These domains have been shown to bind extracellular matrix proteins, an interaction that explains the importance of this collagen in organizing matrix components. Mutations in this gene are associated with Bethlem myopathy and Ullrich scleroatonic muscular dystrophy. Three transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Collagen VI alpha 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55141

GTX55141 ICC/IF Image

ICC/IF analysis of formalin-fixed HT29 cells using GTX55141 Collagen VI alpha 2 antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-Collagen VI alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX55141

GTX55141 IHC-P Image

IHC-P analysis of formalin fixed human lung cancer tissue section using GTX55141 Collagen VI alpha 2 antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-Collagen VI alpha 2 antibody used in Western Blot (WB). GTX55141

GTX55141 WB Image

WB analysis of HepG2 (A), HT29 (B) whole cell lysates using GTX55141 Collagen VI alpha 2 antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399