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Creatine kinase MT antibody

Anti-Creatine kinase MT antibody used in IHC (Paraffin sections) (IHC-P). GTX103627
Anti-Creatine kinase MT antibody used in Western Blot (WB). GTX103627

Cat No. GTX103627

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , H1299 , HeLaS3 , HepG2

Predict Reactivity

Rat, Rabbit, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Creatine kinase MT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CKMT antibody, CKMT1 antibody, CKMT1A antibody, UMTCK antibody, CKMT1B antibody, "creatine kinase U-type, mitochondrial antibody", "creatine kinase, mitochondrial 1 (ubiquitous) antibody", ubiquitous mitochondrial creatine kinase antibody, U-MtCK antibody, acidic-type mitochondrial creatine kinase antibody, mia-CK antibody, "creatine kinase, mitochondrial 1B antibody"

Cellular Localization

Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side

Background

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Creatine kinase MT antibody used in IHC (Paraffin sections) (IHC-P). GTX103627

GTX103627 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Creatine kinase 1B(GTX103627) antibody at 1:300 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Creatine kinase MT antibody used in Western Blot (WB). GTX103627

GTX103627 WB Image

Sample(30 ug whole cell lysate)
A:H1299
B:HeLa S3(GTX14654)
10% SDS PAGE
GTX103627 diluted at 1:1000

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169