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CrkL (phospho Tyr207) antibody

Orthogonal
Anti-CrkL (phospho Tyr207) antibody used in Western Blot (WB). GTX133949
Anti-CrkL (phospho Tyr207) antibody used in Western Blot (WB). GTX133949
Anti-CrkL (phospho Tyr207) antibody used in Western Blot (WB). GTX133949

Cat. No. GTX133949

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CrkL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

K562 , Neuro2A , NIH-3T3 , C2C12 , Rat2

Predict Reactivity

Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr207 of human CrkL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887151

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CRK like proto-oncogene, adaptor protein

Background

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.

Database

Research Area

DATA IMAGES

Anti-CrkL (phospho Tyr207) antibody used in Western Blot (WB). GTX133949

GTX133949 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CrkL (phospho Tyr207) antibody (GTX133949) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CrkL (phospho Tyr207) antibody used in Western Blot (WB). GTX133949

GTX133949 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CrkL (phospho Tyr207) antibody (GTX133949) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CrkL (phospho Tyr207) antibody used in Western Blot (WB). GTX133949

GTX133949 WB Image

Orthogonal

Untreated (–) and treated (+) K562 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CrkL (phospho Tyr207) antibody (GTX133949) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169