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Cullin 4a antibody [JU07-33]

Recombinant antibody
Anti-Cullin 4a antibody [JU07-33] used in Western Blot (WB). GTX01050
Anti-Cullin 4a antibody [JU07-33] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01050
Anti-Cullin 4a antibody [JU07-33] used in IHC (Paraffin sections) (IHC-P). GTX01050
Anti-Cullin 4a antibody [JU07-33] used in Flow cytometry (FACS). GTX01050
Anti-Cullin 4a antibody [JU07-33] used in IHC (Paraffin sections) (IHC-P). GTX01050
Anti-Cullin 4a antibody [JU07-33] used in IHC (Paraffin sections) (IHC-P). GTX01050
Anti-Cullin 4a antibody [JU07-33] used in IHC (Paraffin sections) (IHC-P). GTX01050
Anti-Cullin 4a antibody [JU07-33] used in IHC (Paraffin sections) (IHC-P). GTX01050
Anti-Cullin 4a antibody [JU07-33] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01050

Cat. No. GTX01050

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 JU07-33

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($399)
See all Cullin 4a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cullin 4A , CUL4A , Cullin 4a

Background

CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins (Liu et al., 2009 [PubMed 19481525]).[supplied by OMIM, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-Cullin 4a antibody [JU07-33] used in Western Blot (WB). GTX01050

GTX01050 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cullin 4a antibody [JU07-33] (GTX01050) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The double bands were observed. It is possibly due to post translational modification.
Reference: PMID: 24870930 and PMID: 21487042

Anti-Cullin 4a antibody [JU07-33] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01050

GTX01050 ICC/IF Image

ICC/IF analysis of PFA-fixed HUVEC cells using GTX01050 Cullin 4a antibody [JU07-33].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-Cullin 4a antibody [JU07-33] used in IHC (Paraffin sections) (IHC-P). GTX01050

GTX01050 IHC-P Image

IHC-P analysis of human kidney tissue section using GTX01050 Cullin 4a antibody [JU07-33].

Anti-Cullin 4a antibody [JU07-33] used in Flow cytometry (FACS). GTX01050

GTX01050 FACS Image

FACS analysis of HeLa cells using GTX01050 Cullin 4a antibody [JU07-33].
Green : primary antibody
Red : unlabelled control
Dilution : 1:100

Anti-Cullin 4a antibody [JU07-33] used in IHC (Paraffin sections) (IHC-P). GTX01050

GTX01050 IHC-P Image

IHC-P analysis of human cervix tissue section using GTX01050 Cullin 4a antibody [JU07-33].

Anti-Cullin 4a antibody [JU07-33] used in IHC (Paraffin sections) (IHC-P). GTX01050

GTX01050 IHC-P Image

IHC-P analysis of human colon cancer tissue section using GTX01050 Cullin 4a antibody [JU07-33].

Anti-Cullin 4a antibody [JU07-33] used in IHC (Paraffin sections) (IHC-P). GTX01050

GTX01050 IHC-P Image

IHC-P analysis of human liver cancer tissue section using GTX01050 Cullin 4a antibody [JU07-33].

Anti-Cullin 4a antibody [JU07-33] used in IHC (Paraffin sections) (IHC-P). GTX01050

GTX01050 IHC-P Image

IHC-P analysis of human breast cancer tissue section using GTX01050 Cullin 4a antibody [JU07-33].

Anti-Cullin 4a antibody [JU07-33] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01050

GTX01050 ICC/IF Image

ICC/IF analysis of PFA-fixed A431 cells using GTX01050 Cullin 4a antibody [JU07-33].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399