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Cyclin A1 antibody

Anti-Cyclin A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02524
Anti-Cyclin A1 antibody used in Western Blot (WB). GTX02524
Anti-Cyclin A1 antibody used in Western Blot (WB). GTX02524
Anti-Cyclin A1 antibody used in Western Blot (WB). GTX02524

Cat. No. GTX02524

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($469)
See all Cyclin A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Cyclin A1 (aa 411-460).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin A1 , CT146

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cyclin A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02524

GTX02524 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX02524 Cyclin A1 antibody. The picture on the right is blocked with the synthesized peptide.

Anti-Cyclin A1 antibody used in Western Blot (WB). GTX02524

GTX02524 WB Image

WB analysis of various cell lysates using GTX02524 Cyclin A1 antibody.
Dilution : 1:2000

Anti-Cyclin A1 antibody used in Western Blot (WB). GTX02524

GTX02524 WB Image

WB analysis of HeLa cell lysate using GTX02524 Cyclin A1 antibody.
Dilution : 1:2000

Anti-Cyclin A1 antibody used in Western Blot (WB). GTX02524

GTX02524 WB Image

WB analysis of SKOV3 cell lysate using GTX02524 Cyclin A1 antibody. The lane on the right is blocked with the synthesized peptide.

REFERENCE

Application Reference

Hsieh CC et al. Biomedicines 2023; 11 (10) Allyl Isothiocyanate Suppresses the Proliferation in Oral Squamous Cell Carcinoma via Mediating the KDM8/CCNA1 Axis.
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REVIEW

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Package List Price ($)
$ 469