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Cyclin A2 antibody [GT2547]

Anti-Cyclin A2 antibody [GT2547] used in IHC (Paraffin sections) (IHC-P). GTX634420
Anti-Cyclin A2 antibody [GT2547] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634420
Anti-Cyclin A2 antibody [GT2547] used in IHC (Paraffin sections) (IHC-P). GTX634420
Anti-Cyclin A2 antibody [GT2547] used in IHC (Paraffin sections) (IHC-P). GTX634420
Anti-Cyclin A2 antibody [GT2547] used in Western Blot (WB). GTX634420

Cat. No. GTX634420

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT2547

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Cyclin A2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Rabbit, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cyclin A2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888449

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin A2 , CCN1 , CCNA

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cyclin A2 antibody [GT2547] used in IHC (Paraffin sections) (IHC-P). GTX634420

GTX634420 IHC-P Image

Cyclin A2 antibody [GT2547] detects Cyclin A2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
Cyclin A2 stained by Cyclin A2 antibody [GT2547] (GTX634420) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cyclin A2 antibody [GT2547] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634420

GTX634420 ICC/IF Image

Cyclin A2 antibody [GT2547] detects Cyclin A2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cyclin A2 stained by Cyclin A2 antibody [GT2547] (GTX634420) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-Cyclin A2 antibody [GT2547] used in IHC (Paraffin sections) (IHC-P). GTX634420

GTX634420 IHC-P Image

Cyclin A2 antibody [GT2547] detects Cyclin A2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Cyclin A2 stained by Cyclin A2 antibody [GT2547] (GTX634420) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cyclin A2 antibody [GT2547] used in IHC (Paraffin sections) (IHC-P). GTX634420

GTX634420 IHC-P Image

Cyclin A2 antibody [GT2547] detects Cyclin A2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Cyclin A2 stained by Cyclin A2 antibody [GT2547] (GTX634420) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cyclin A2 antibody [GT2547] used in Western Blot (WB). GTX634420

GTX634420 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody [GT2547] (GTX634420) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Dai J et al. Nat Commun 2024; 15 (1) : 1158 Tracing back primed resistance in cancer via sister cells.
Valles SY et al. bioRxiv 2023; Cyclin A/Cdk1 promotes chromosome alignment and timely mitotic progression.
D Larrieu et al. Sci Rep. 2020; 10 (1) : 789 The E3 ubiquitin ligase TRIP12 participates in cell cycle progression and chromosome stability.
Application : ICC/IF
Reactivity : Human
Andrea Maria Guarino et al. Genes 2018; 9 (10) : 513 Oxidative Stress Causes Enhanced Secretion of YB-1 Protein that Restrains Proliferation of Receiving Cells.
Application : WB
Reactivity : Human
Ming-YangLee et al. European Journal of Pharmacology 2018; 839 : 57-65 Indirubin-3'-oxime suppresses human cholangiocarcinoma through cell-cycle arrest and apoptosis.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations