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Cyclin D2 antibody

Anti-Cyclin D2 antibody used in IHC (Paraffin sections) (IHC-P). GTX32545
Anti-Cyclin D2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32545
Anti-Cyclin D2 antibody used in Western Blot (WB). GTX32545
Anti-Cyclin D2 antibody used in IHC (Paraffin sections) (IHC-P). GTX32545

Cat. No. GTX32545

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Cyclin D2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human CCND2 (NP_001750.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin D2 , KIAK0002 , MPPH3

Cellular Localization

Nucleus,Cytoplasm,Membrane

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK4 or CDK6 and functions as a regulatory subunit of the complex, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. Mutations in this gene are associated with megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 3 (MPPH3). [provided by RefSeq, Sep 2014]

Database

Research Area

DATA IMAGES

Anti-Cyclin D2 antibody used in IHC (Paraffin sections) (IHC-P). GTX32545

GTX32545 IHC-P Image

IHC-P analysis of human stomach tissue using GTX32545 Cyclin D2 antibody.
Dilution : 1:100

Anti-Cyclin D2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32545

GTX32545 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32545 Cyclin D2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-Cyclin D2 antibody used in Western Blot (WB). GTX32545

GTX32545 WB Image

WB analysis of various sample lysates using GTX32545 Cyclin D2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Cyclin D2 antibody used in IHC (Paraffin sections) (IHC-P). GTX32545

GTX32545 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX32545 Cyclin D2 antibody.
Dilution : 1:100

REFERENCE

Application Reference

D?az-L?pez YE et al. FEBS Open Bio 2023; 13 (12) : 2263-2272 ETV5 regulates proliferation and cell cycle genes in the INS-1 (832/13) cell line independently of the concentration of secreted insulin.
Wenbiao Chen et al. Cell Commun Signal 2022; 20 (1) : 28 Ameliorating role of microRNA-378 carried by umbilical cord mesenchymal stem cells-released extracellular vesicles in mesangial proliferative glomerulonephritis.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399