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Cyclin T1 antibody

Anti-Cyclin T1 antibody used in Western Blot (WB). GTX133413
Anti-Cyclin T1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133413
Anti-Cyclin T1 antibody used in Western Blot (WB). GTX133413
Anti-Cyclin T1 antibody used in Western Blot (WB). GTX133413

Cat. No. GTX133413

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Cyclin T1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cyclin T1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886973

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin T1 , CCNT , CYCT1 , HIVE1

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin tightly associates with CDK9 kinase, and was found to be a major subunit of the transcription elongation factor p-TEFb. The kinase complex containing this cyclin and the elongation factor can interact with, and act as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and was shown to be both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner were also found to be involved in the phosphorylation and regulation of the carboxy-terminal domain (CTD) of the largest RNA polymerase II subunit. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cyclin T1 antibody used in Western Blot (WB). GTX133413

GTX133413 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cyclin T1 antibody (GTX133413) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin T1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133413

GTX133413 ICC/IF Image

Cyclin T1 antibody detects Cyclin T1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cyclin T1 protein stained by Cyclin T1 antibody (GTX133413) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Anti-Cyclin T1 antibody used in Western Blot (WB). GTX133413

GTX133413 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cyclin T1 antibody (GTX133413) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin T1 antibody used in Western Blot (WB). GTX133413

GTX133413 WB Image

Various whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cyclin T1 antibody (GTX133413) diluted at 1:1000.

REFERENCE

Application Reference

N Neville et al. Cell Rep 2023; 42 (9) : 113082 Modification of histidine repeat proteins by inorganic polyphosphate
Application : WB
Luo Y et al. bioRxiv 2023; mRNA interactions with disordered regions control protein activity.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169