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Cystatin S antibody [N1N3]

Anti-Cystatin S antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100690
Anti-Cystatin S antibody [N1N3] used in Western Blot (WB). GTX100690
Anti-Cystatin S antibody [N1N3] used in Western Blot (WB). GTX100690
Anti-Cystatin S antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX100690

Cat. No. GTX100690

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Cystatin S products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Cystatin S. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950052

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cystatin S

Cellular Localization

Secreted

Background

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a type 2 salivary cysteine peptidase inhibitor. The protein is an S-type cystatin, based on its high level of expression in saliva, tears and seminal plasma. The specific role in these fluids is unclear but antibacterial and antiviral activity is present, consistent with a protective function. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cystatin S antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100690

GTX100690 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using Cystatin S(GTX100690) antibody at 1:200 dilution.

Anti-Cystatin S antibody [N1N3] used in Western Blot (WB). GTX100690

GTX100690 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cystatin S antibody [N1N3] (GTX100690) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cystatin S antibody [N1N3] used in Western Blot (WB). GTX100690

GTX100690 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cystatin S antibody [N1N3] (GTX100690) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cystatin S antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX100690

GTX100690 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Cystatin S(GTX100690) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Maruyama Y et al. PeerJ 2023; 11 : e15075 Analysis of IGHA1 and other salivary proteins post half marathon in female participants.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169