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Cytokeratin 13 antibody [3G4]

Anti-Cytokeratin 13 antibody [3G4] used in Flow cytometry (FACS). GTX60622
Anti-Cytokeratin 13 antibody [3G4] used in Western Blot (WB). GTX60622
Anti-Cytokeratin 13 antibody [3G4] used in ELISA (ELISA). GTX60622

Cat. No. GTX60622

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3G4

Isotype

 IgG1

Application

 WB, FACS, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all Cytokeratin 13 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human Cytokeratin 13 (AA: 143-295) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 13 , CK13 , K13 , WSN2

Background

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 13 antibody [3G4] used in Flow cytometry (FACS). GTX60622

GTX60622 FACS Image

FACS analysis of HeLa cells using GTX60622 Cytokeratin 13 antibody [3G4].
Green : Cytokeratin 13
Red : negative control

Anti-Cytokeratin 13 antibody [3G4] used in Western Blot (WB). GTX60622

GTX60622 WB Image

WB analysis of T47D cell lysate using GTX60622 Cytokeratin 13 antibody [3G4].

Anti-Cytokeratin 13 antibody [3G4] used in ELISA (ELISA). GTX60622

GTX60622 ELISA Image

ELISA analysis of antigen using GTX60622 Cytokeratin 13 antibody [3G4].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399