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Cytokeratin 14 antibody [N3C3]

Anti-Cytokeratin 14 antibody [N3C3] used in Western Blot (WB). GTX112976
Anti-Cytokeratin 14 antibody [N3C3] used in Western Blot (WB). GTX112976
Anti-Cytokeratin 14 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112976

Cat. No. GTX112976

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Dog
Package
100 μl ($399),
25 μl ($169)
See all Cytokeratin 14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729205

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 14 , CK14 , EBS3 , EBS4 , K14 , NFJ

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 14 antibody [N3C3] used in Western Blot (WB). GTX112976

GTX112976 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 14 antibody (GTX112976) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 14 antibody [N3C3] used in Western Blot (WB). GTX112976

GTX112976 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX112976 diluted at 1:1000

Anti-Cytokeratin 14 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112976

GTX112976 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using Cytokeratin 14(GTX112976) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169