APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-2μg/ml |
Assay dependent |
1-2μg/ml for 30 min at RT |
Assay dependent |
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
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Not tested in other applications.
Calculated MW
Product Note
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.
We do not recommend use of this product for Rat,Sheep,Bovine,Dog,Hamster,Pig samples.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human KRT18 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CK18 , CYK18 , K18 , KRT18 , keratin 18 , Cytokeratin 18
Cellular Localization
Cytoplasm,Perinuclear region,Nucleus,Nucleolus
Background
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX34643 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX34643 Cytokeratin 18 antibody [KRT18/835].
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GTX34643 IHC-P Image
IHC-P analysis of human thyroid cancer tissue using GTX34643 Cytokeratin 18 antibody [KRT18/835].
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GTX34643 IHC-P Image
IHC-P analysis of human prostate cancer tissue using GTX34643 Cytokeratin 18 antibody [KRT18/835].
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GTX34643 WB Image
WB analysis of HCT116 cell lysate using GTX34643 Cytokeratin 18 antibody [KRT18/835].
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GTX34643 IHC-P Image
IHC-P analysis of human lung cancer tissue using GTX34643 Cytokeratin 18 antibody [KRT18/835].
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GTX34643 Protein Array Image
Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/835). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
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GTX34643 IHC-P Image
IHC-P analysis of human bladder carcinoma tissue using GTX34643 Cytokeratin 18 antibody [KRT18/835].
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GTX34643 ICC/IF Image
ICC/IF analysis of MCF cells using GTX34643 Cytokeratin 18 antibody [KRT18/835]. Green : Primary antibody Red : nucleus
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REFERENCE
There are currently no references for Cytokeratin 18 antibody [KRT18/835] (GTX34643). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cytokeratin 18 antibody [KRT18/835] (GTX34643). Be the first to share your experience with this product.