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Cytokeratin 19 antibody [3F8]

Overexpression
Anti-Cytokeratin 19 antibody [3F8] used in Western Blot (WB). GTX84251
Anti-Cytokeratin 19 antibody [3F8] used in IHC (Paraffin sections) (IHC-P). GTX84251
Anti-Cytokeratin 19 antibody [3F8] used in IHC (Paraffin sections) (IHC-P). GTX84251
Anti-Cytokeratin 19 antibody [3F8] used in IHC (Paraffin sections) (IHC-P). GTX84251
Anti-Cytokeratin 19 antibody [3F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84251
Anti-Cytokeratin 19 antibody [3F8] used in IHC (Paraffin sections) (IHC-P). GTX84251
Anti-Cytokeratin 19 antibody [3F8] used in IHC (Paraffin sections) (IHC-P). GTX84251
Anti-Cytokeratin 19 antibody [3F8] used in Western Blot (WB). GTX84251
Anti-Cytokeratin 19 antibody [3F8] used in IHC (Paraffin sections) (IHC-P). GTX84251
Anti-Cytokeratin 19 antibody [3F8] used in IHC (Paraffin sections) (IHC-P). GTX84251
Anti-Cytokeratin 19 antibody [3F8] used in IHC (Paraffin sections) (IHC-P). GTX84251

Cat. No. GTX84251

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3F8

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat, Dog, Monkey
Package
100 μl ($509)
See all Cytokeratin 19 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
IHC-P 1:50-1:150
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment expressed in E.coli corresponding to amino acids 240-390 of human CK19

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 19 , CK19 , K19 , K1CS

Background

Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 19 antibody [3F8] used in Western Blot (WB). GTX84251

GTX84251 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Cytokeratin 19 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84251 Cytokeratin 19 antibody [3F8].
Loading : 5 ug per lane

Anti-Cytokeratin 19 antibody [3F8] used in IHC (Paraffin sections) (IHC-P). GTX84251

GTX84251 IHC-P Image

IHC-P analysis of liver tissue using GTX84251 Cytokeratin 19 antibody [3F8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Cytokeratin 19 antibody [3F8] used in IHC (Paraffin sections) (IHC-P). GTX84251

GTX84251 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84251 Cytokeratin 19 antibody [3F8].
Dilution : 1:150

Anti-Cytokeratin 19 antibody [3F8] used in IHC (Paraffin sections) (IHC-P). GTX84251

GTX84251 IHC-P Image

IHC-P analysis of human bladder carcinoma tissue using GTX84251 Cytokeratin 19 antibody [3F8].
Dilution : 1:150

Anti-Cytokeratin 19 antibody [3F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84251

GTX84251 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Cytokeratin 19 plasmid using GTX84251 Cytokeratin 19 antibody [3F8].

Anti-Cytokeratin 19 antibody [3F8] used in IHC (Paraffin sections) (IHC-P). GTX84251

GTX84251 IHC-P Image

IHC-P analysis of endometrium adenocarcinoma tissue using GTX84251 Cytokeratin 19 antibody [3F8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Cytokeratin 19 antibody [3F8] used in IHC (Paraffin sections) (IHC-P). GTX84251

GTX84251 IHC-P Image

IHC-P analysis of human bladder tissue using GTX84251 Cytokeratin 19 antibody [3F8].
Dilution : 1:150

Anti-Cytokeratin 19 antibody [3F8] used in Western Blot (WB). GTX84251

GTX84251 WB Image

WB analysis of various cell lines using GTX84251 Cytokeratin 19 antibody [3F8].
Loading : 35 ug per lane

Anti-Cytokeratin 19 antibody [3F8] used in IHC (Paraffin sections) (IHC-P). GTX84251

GTX84251 IHC-P Image

IHC-P analysis of kidney tissue using GTX84251 Cytokeratin 19 antibody [3F8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Cytokeratin 19 antibody [3F8] used in IHC (Paraffin sections) (IHC-P). GTX84251

GTX84251 IHC-P Image

IHC-P analysis of human colon tissue using GTX84251 Cytokeratin 19 antibody [3F8].
Dilution : 1:150

Anti-Cytokeratin 19 antibody [3F8] used in IHC (Paraffin sections) (IHC-P). GTX84251

GTX84251 IHC-P Image

IHC-P analysis of kidney carcinoma tissue using GTX84251 Cytokeratin 19 antibody [3F8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 509