Cytokeratin 5 antibody [AT12C4]

Cat. No. GTX57627

Host	Mouse
Clonality	Monoclonal
Clone Name	AT12C4
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human
Package	100 μl ($399)

See all Cytokeratin 5 products

Datasheet
Document

View product citations for antibody GTX57627 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-P	Assay dependent
FACS	Assay dependent

Not tested in other applications.

Calculated MW

62 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT12C4 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human KRT7 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 5 , CK5 , DDD , DDD1 , EBS2 , K5 , KRT5 , KRT5A , Cytokeratin 5

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]

Database

Gene ID: 3852 KRT5
UniProt: P13647 KRT5

Research Area

DATA IMAGES

GTX57627 FACS Image

FACS analysis of HeLa cells using GTX57627 Cytokeratin 5 antibody.
Cell Number: 1 x 10⁶ cells
Red: Primary antibody
Antibody amount: 2-5 μg

GTX57627 ICC/IF Image

ICC/IF analysis of A549 cells using GTX57627 Cytokeratin 5 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

GTX57627 WB Image

WB analysis of various samples using GTX57627 Cytokeratin 5 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : A431 whole cell lysate
Loading : 40 μg
Dilution : 1:1000

GTX57627 IHC-P Image

IHC-P analysis of human cervical cancer using GTX57627 Cytokeratin 5 antibody.
Antigen retrieval: 0.1M sodium citrate buffer
Dilution: 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty