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Cytokeratin 8 antibody [B22.1]

Anti-Cytokeratin 8 antibody [B22.1] used in IHC (Paraffin sections) (IHC-P). GTX34664
Anti-Cytokeratin 8 antibody [B22.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34664
Anti-Cytokeratin 8 antibody [B22.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34664
Anti-Cytokeratin 8 antibody [B22.1] used in Western Blot (WB). GTX34664

Cat. No. GTX34664

Host

Mouse

Clonality

Monoclonal

Clone Name

B22.1

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Rabbit, Sheep, Bovine, Pig
Package
100 μg ($529)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 min at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Product Note

We do not recommend use of this product for Mouse,Rat,Chicken,Xenopus laevis samples.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Cytoskeletal preparation from HeLa cells

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CARD2 , CK8 , CYK8 , K2C8 , K8 , KO , KRT8 , keratin 8 , Cytokeratin 8 , Keratin 8

Cellular Localization

Cytoplasm,Nucleus,Nucleoplasm,Nucleus matrix

Background

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 8 antibody [B22.1] used in IHC (Paraffin sections) (IHC-P). GTX34664

GTX34664 IHC-P Image

IHC-P analysis of colon carcinoma tissue using GTX34664 Cytokeratin 8 antibody [B22.1].

Anti-Cytokeratin 8 antibody [B22.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34664

GTX34664 ICC/IF Image

ICC/IF analysis of MCF cells using GTX34664 Cytokeratin 8 antibody [B22.1].
Green : Primary antibody
Red : nucleus

Anti-Cytokeratin 8 antibody [B22.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34664

GTX34664 ICC/IF Image

ICC/IF analysis of MCF cells using GTX34664 Cytokeratin 8 antibody [B22.1].
Green : Primary antibody
Red : nucleus

Anti-Cytokeratin 8 antibody [B22.1] used in Western Blot (WB). GTX34664

GTX34664 WB Image

WB analysis of HCT116 cell lysate using GTX34664 Cytokeratin 8 antibody [B22.1].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529