APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml |
| 1-2μg/ml for 30 min at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Cytoskeleton preparation containing cytokeratin 8
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CARD2 , CK8 , CYK8 , K2C8 , K8 , KO , KRT8 , keratin 8 , Cytokeratin 8 , Keratin 8
Cellular Localization
Cytoplasm,Nucleus,Nucleoplasm,Nucleus matrix
Background
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
Database
Research Area
DATA IMAGES
![Anti-Cytokeratin 8 antibody [H1] used in Western Blot (WB). GTX34663](/templates/default/js/lightbox/images/loading.gif "Anti-Cytokeratin 8 antibody [H1] used in Western Blot (WB). GTX34663")
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GTX34663 WB Image
WB analysis of A431 cell lysate using GTX34663 Cytokeratin 8 antibody [H1].
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GTX34663 ICC/IF Image
ICC/IF analysis of HCT116 cells using GTX34663 Cytokeratin 8 antibody [H1].
Green : Primary antibody
Red : nucleus
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GTX34663 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX34663 Cytokeratin 8 antibody [H1].
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GTX34663 FACS Image
FACS analysis of HeLa cells using GTX34663 Cytokeratin 8 antibody [H1].
Blue : Primary antibody
Red : Isotype control
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GTX34663 ICC/IF Image
ICC/IF analysis of MCF-7 cells using GTX34663 Cytokeratin 8 antibody [H1].
Green : Primary antibody
Red : nucleus
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REFERENCE
There are currently no references for Cytokeratin 8 antibody [H1] (GTX34663). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cytokeratin 8 antibody [H1] (GTX34663). Be the first to share your experience with this product.
![Anti-Cytokeratin 8 antibody [H1] used in Western Blot (WB). GTX34663](/upload/website/prouct_img/small/GTX34663/GTX34663_20200115_WB_35_w_23060801_306.webp "Anti-Cytokeratin 8 antibody [H1] used in Western Blot (WB). GTX34663")
![Anti-Cytokeratin 8 antibody [H1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34663](/upload/website/prouct_img/small/GTX34663/GTX34663_20200115_ICC-IF_91_w_23060801_961.webp "Anti-Cytokeratin 8 antibody [H1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34663")
![Anti-Cytokeratin 8 antibody [H1] used in IHC (Paraffin sections) (IHC-P). GTX34663](/upload/website/prouct_img/small/GTX34663/GTX34663_20200115_IHC-P_458_w_23060801_599.webp "Anti-Cytokeratin 8 antibody [H1] used in IHC (Paraffin sections) (IHC-P). GTX34663")
![Anti-Cytokeratin 8 antibody [H1] used in Flow cytometry (FACS). GTX34663](/upload/website/prouct_img/small/GTX34663/GTX34663_20200115_FACS_182_w_23060801_931.webp "Anti-Cytokeratin 8 antibody [H1] used in Flow cytometry (FACS). GTX34663")
![Anti-Cytokeratin 8 antibody [H1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34663](/upload/website/prouct_img/small/GTX34663/GTX34663_20200115_ICC-IF_1571_w_23060801_982.webp "Anti-Cytokeratin 8 antibody [H1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34663")
![Anti-Cytokeratin 8 antibody [H1] used in Western Blot (WB). GTX34663](/upload/website/prouct_img/normal/GTX34663/GTX34663_20200115_WB_35_w_23060801_306.webp "Anti-Cytokeratin 8 antibody [H1] used in Western Blot (WB). GTX34663")
![Anti-Cytokeratin 8 antibody [H1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34663](/upload/website/prouct_img/normal/GTX34663/GTX34663_20200115_ICC-IF_91_w_23060801_961.webp "Anti-Cytokeratin 8 antibody [H1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34663")
![Anti-Cytokeratin 8 antibody [H1] used in IHC (Paraffin sections) (IHC-P). GTX34663](/upload/website/prouct_img/normal/GTX34663/GTX34663_20200115_IHC-P_458_w_23060801_599.webp "Anti-Cytokeratin 8 antibody [H1] used in IHC (Paraffin sections) (IHC-P). GTX34663")
![Anti-Cytokeratin 8 antibody [H1] used in Flow cytometry (FACS). GTX34663](/upload/website/prouct_img/normal/GTX34663/GTX34663_20200115_FACS_182_w_23060801_931.webp "Anti-Cytokeratin 8 antibody [H1] used in Flow cytometry (FACS). GTX34663")
![Anti-Cytokeratin 8 antibody [H1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34663](/upload/website/prouct_img/normal/GTX34663/GTX34663_20200115_ICC-IF_1571_w_23060801_982.webp "Anti-Cytokeratin 8 antibody [H1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34663")