APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-1μg/ml |
| 5μg/ml |
| 10μg/ml |
|
Note :
IHC-P
Human Kidney stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89467-PEP).
Product Note
This antibody is expected to recognize both reported isoforms (NP_001116423.1; NP_002027.2).
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence HRAELSPSTENSSQLDFED-C, from the N Terminus of the protein sequence according to NP_001116423.1; NP_002027.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
atypical chemokine receptor 1 (Duffy blood group) , CCBP1 , CD234 , DARC , DARC/ACKR1 , Dfy , FY , GPD , GpFy , WBCQ1
Cellular Localization
Membrane; Multi-pass membrane protein
Background
The protein encoded by this gene is a glycosylated membrane protein and a non-specific receptor for several chemokines. The encoded protein is the receptor for the human malarial parasites Plasmodium vivax and Plasmodium knowlesi. Polymorphisms in this gene are the basis of the Duffy blood group system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX89467 FACS Image
FACS analysis of PFA fixed HEK293 cells using GTX89467 DARC antibody, N-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml
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GTX89467 IHC-P Image
IHC-P analysis of human kidney using GTX89467 DARC antibody, N-term.
Antigen retrieval : citrate buffer Ph 6
Dilution : 3μg/ml
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GTX89467 WB Image
WB analysis of human liver lysate using GTX89467 DARC antibody, N-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89467 IHC-P Image
IHC-P analysis of human lung using GTX89467 DARC antibody, N-term.
Antigen retrieval : citrate buffer Ph 6
Dilution : 5μg/ml
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REFERENCE
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REVIEW
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