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DCDC2 antibody, Internal

Anti-DCDC2 antibody, Internal used in Western Blot (WB). GTX89389
Anti-DCDC2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89389

Cat. No. GTX89389

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all DCDC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
IHC-P 5-10μg/ml

Note :

IHC-P
Human Cerebral Cortex shows cytoplasm staining in select neurons.
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89389-PEP).

Predict Reactivity

Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DGEKANKDAEQKED, from the internal region of the protein sequence according to NP_057440.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

doublecortin domain containing 2 , DCDC2A , DFNB66 , NPHP19 , NSC , RU2 , RU2S

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a doublecortin domain-containing family member. The doublecortin domain has been demonstrated to bind tubulin and enhance microtubule polymerization. This family member is thought to function in neuronal migration where it may affect the signaling of primary cilia. Mutations in this gene have been associated with reading disability (RD) type 2, also referred to as developmental dyslexia. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2013]

Database

Research Area

DATA IMAGES

Anti-DCDC2 antibody, Internal used in Western Blot (WB). GTX89389

GTX89389 WB Image

WB analysis of COS7 cell lysate transfected with full length recombinant human DCDC2 (A) and untransfected control COS7 cells (B) using GTX89389 DCDC2 antibody, Internal.
Dilution : 0.2μg/ml

Anti-DCDC2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89389

GTX89389 IHC-P Image

IHC-P analysis of human cerebral cortex using GTX89389 DCDC2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399