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DCK antibody

KO/KD Overexpression
Anti-DCK antibody used in Western Blot (WB). GTX102800
Anti-DCK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102800
Anti-DCK antibody used in Western Blot (WB). GTX102800
Anti-DCK antibody used in Western Blot (WB). GTX102800
Anti-DCK antibody used in IHC (Paraffin sections) (IHC-P). GTX102800
Anti-DCK antibody used in Western Blot (WB). GTX102800
Anti-DCK antibody used in Western Blot (WB). GTX102800
Anti-DCK antibody used in IHC (Paraffin sections) (IHC-P). GTX102800
Anti-DCK antibody used in Immunoprecipitation (IP). GTX102800
Anti-DCK antibody used in Western Blot (WB). GTX102800
Anti-DCK antibody used in Western Blot (WB). GTX102800
Anti-DCK antibody used in Immunohistochemistry (IHC). GTX102800
Anti-DCK antibody used in Immunohistochemistry (IHC). GTX102800

Cat. No. GTX102800

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all DCK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
IHC Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

PC-12 , Rat2 , A549 , H1299 , 293T , DCK-transfected 293T

Predict Reactivity

Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DCK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163443

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

deoxycytidine kinase

Cellular Localization

Nucleus

Background

Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DCK antibody used in Western Blot (WB). GTX102800

GTX102800 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (GTX102800) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DCK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102800

GTX102800 ICC/IF Image

DCK antibody detects DCK protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DCK protein stained by DCK antibody (GTX102800) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

Anti-DCK antibody used in Western Blot (WB). GTX102800

GTX102800 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (GTX102800) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DCK antibody used in Western Blot (WB). GTX102800

GTX102800 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (GTX102800) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DCK antibody used in IHC (Paraffin sections) (IHC-P). GTX102800

GTX102800 IHC-P Image

DCK antibody detects DCK protein at nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
DCK antibody (GTX102800) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DCK antibody used in Western Blot (WB). GTX102800

GTX102800 WB Image

DCK antibody detects DCK protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
12% SDS-PAGE
DCK antibody (GTX102800) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DCK antibody used in Western Blot (WB). GTX102800

GTX102800 WB Image

DCK antibody detects DCK protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (GTX102800) diluted at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DCK antibody used in IHC (Paraffin sections) (IHC-P). GTX102800

GTX102800 IHC-P Image

DCK antibody detects DCK protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
DCK stained by DCK antibody (GTX102800) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DCK antibody used in Immunoprecipitation (IP). GTX102800

GTX102800 IP Image

Immunoprecipitation of DCK protein from 293T whole cell extracts using 5 μg of DCK antibody (GTX102800).
Western blot analysis was performed using DCK antibody (GTX102800).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-DCK antibody used in Western Blot (WB). GTX102800

GTX102800 WB Image

KO/KD

The data was published in the journal Sci Rep in 2019. PMID: 31113993

Anti-DCK antibody used in Western Blot (WB). GTX102800

GTX102800 WB Image

KO/KD

The data was published in the journal Sci Rep in 2019. PMID: 31113993

Anti-DCK antibody used in Immunohistochemistry (IHC). GTX102800

GTX102800 IHC Image

The data was published in the journal Sci Rep in 2019. PMID: 31113993

Anti-DCK antibody used in Immunohistochemistry (IHC). GTX102800

GTX102800 IHC Image

The data was published in the journal Sci Rep in 2019. PMID: 31113993

REFERENCE

Application Reference

Dash S et al. Cell Death Dis 2024; 15 (2) : 131 Deoxycytidine kinase inactivation enhances gemcitabine resistance and sensitizes mitochondrial metabolism interference in pancreatic cancer.
Suksawat M et al. PLoS One 2019; 14 (9) : e0222140 In vitro and molecular chemosensitivity in human cholangiocarcinoma tissues.
Application : ICC/IF
Reactivity : Human
Sarr A et al. Sci Rep 2019; 9 (1) : 7643 Genome-scale CRISPR/Cas9 screen determines factors modulating sensitivity to ProTide NUC-1031.
Application : WB
Reactivity : Human
Kurata M et al. Sci Rep 2016; 6 : 36199 Using genome-wide CRISPR library screening with library resistant DCK to find new sources of Ara-C drug resistance in AML.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169