APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:10000 |
Assay dependent |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
human DCTD-transfected 293T cells , NT2D1 , PC-3 , U87-MG , SK-N-SH
Predict Reactivity
Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.19 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human DCTD. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885666
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
dCMP deaminase
Background
The protein encoded by this gene catalyzes the deamination of dCMP to dUMP, the nucleotide substrate for thymidylate synthase. The encoded protein is allosterically activated by dCTP and inhibited by dTTP, and is found as a homohexamer. This protein uses zinc as a cofactor for its activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX127971 IHC-P Image
DCTD antibody detects DCTD protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.DCTD stained by DCTD antibody (GTX127971) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX127971 IHC-P Image
DCTD antibody detects DCTD protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. DCTD antibody (GTX127971) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX127971 WB Image
DCTD antibody detects DCTD protein by Western blot analysis. A. 30 μg NT2D1 whole cell lysate/extract B. 30 μg PC-3 whole cell lysate/extract C. 30 μg U87-MG whole cell lysate/extract D. 30 μg SK-N-SH whole cell lysate/extract 12% SDS PAGE DCTD antibody (GTX127971) dilution: 1:1000
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GTX127971 ICC/IF Image
DCTD antibody detects DCTD protein at nucleus, cytoplasm and endoplasmic reticulum by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DCTD stained by DCTD antibody (GTX127971) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX127971 WB Image
DCTD antibody detects DCTD protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of human DCTD-transfected 293T cells 12% SDS PAGE DCTD antibody (GTX127971) dilution: 1:5000
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GTX127971 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCTD antibody (GTX127971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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