APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:2000 |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rabbit, Sheep, Bovine, Dog, Pig, Horse(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human DDX17, corresponding to a region within C-terminal amino acids.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DEAD-box helicase 17 , P72 , RH70
Cellular Localization
Nucleus , Cytoplasm, cytosol , Nucleolus
Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and splicesosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an ATPase activated by a variety of RNA species, but not by dsDNA. This protein, and that encoded by DDX5 gene, are more closely related to each other than to any other member of the DEAD box family. This gene can encode multiple isoforms due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) start codon. [provided by RefSeq, Apr 2011]
Database
Research Area
DATA IMAGES
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GTX02534 ICC/IF Image
ICC/IF analysis of PFA-fixed HeLa cells using GTX02534 DDX17 Antibody. Red : Primary antibody Green : Tubulin Blue : DAPI Permeabilization : 0.1% Triton X-100 Dilution : 1:200
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GTX02534 WB Image
WB analysis of extracts from HEK293 cells using GTX02534 DDX17 Antibody. The lane on the left was treated with blocking peptide.
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GTX02534 WB Image
WB analysis of extracts from HeLa cells using GTX02534 DDX17 Antibody. The lane on the left was treated with blocking peptide.
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REFERENCE
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REVIEW
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