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DDX3 antibody [6G8]

Anti-DDX3 antibody [6G8] used in Western Blot (WB). GTX66840

Cat. No. GTX66840

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6G8

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($459)
See all DDX3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

100mM Tris-Glycine, 150mM NaCl, 50% Glycerol

Preservative

0.2% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human DDX3 protein fragments expressed in E.coli.

Purification

Purified by antigen-affinity chromatography

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DEAD-box helicase 3 X-linked , CAP-Rf , DBX , DDX14 , DDX3 , HLP2 , MRX102

Cellular Localization

Nucleus speckle,Cytoplasm,Mitochondrion outer membrane

Background

The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Database

Research Area

DATA IMAGES

Anti-DDX3 antibody [6G8] used in Western Blot (WB). GTX66840

GTX66840 WB Image

WB analysis of HeLa, 3T3, C6, COS7, K562, and Jurkat cell lysates using GTX66840 DDX3 antibody [6G8].
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459