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DDX58 antibody

Anti-DDX58 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54000
Anti-DDX58 antibody used in IHC (Paraffin sections) (IHC-P). GTX54000
Anti-DDX58 antibody used in Western Blot (WB). GTX54000

Cat. No. GTX54000

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all DDX58 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

107 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide of human DDX58

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DExD/H-box helicase 58 , RIG-I , RIG1 , RIGI , RLR-1 , SGMRT2

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-DDX58 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54000

GTX54000 ICC/IF Image

ICC/IF analysis of L929 cells using GTX54000 DDX58 antibody.
Blue : DAPI
Dilution : 1:100

Anti-DDX58 antibody used in IHC (Paraffin sections) (IHC-P). GTX54000

GTX54000 IHC-P Image

IHC-P analysis of human prostate tissue using GTX54000 DDX58 antibody.
Dilution : 1:100

Anti-DDX58 antibody used in Western Blot (WB). GTX54000

GTX54000 WB Image

WB analysis of various sample lysates using GTX54000 DDX58 antibody.
Dilution : 1:500
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399