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DHCR7 antibody

KO/KD Orthogonal
Anti-DHCR7 antibody used in Western Blot (WB). GTX130695
Anti-DHCR7 antibody used in IHC (Paraffin sections) (IHC-P). GTX130695
Anti-DHCR7 antibody used in IHC (Paraffin sections) (IHC-P). GTX130695
Anti-DHCR7 antibody used in Western Blot (WB). GTX130695

Cat. No. GTX130695

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all DHCR7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Predict Reactivity

Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DHCR7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886322

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

7-dehydrocholesterol reductase , SLOS

Cellular Localization

Endoplasmic reticulum membrane

Background

The DHCR7 gene encodes delta-7-sterol reductase (EC 1.3.1.21), the penultimate enzyme of mammalian sterol biosynthesis that converts 7-dehydrocholesterol (7-DHC) to cholesterol. This enzyme removes the C(7-8) double bond introduced by the sterol delta8-delta7 isomerases. In addition, its role in drug-induced malformations is known: inhibitors of the last step of cholesterol biosynthesis such as AY9944 and BM15766 severely impair brain development (Moebius et al., 1998 [PubMed 9465114]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-DHCR7 antibody used in Western Blot (WB). GTX130695

GTX130695 WB Image

KO/KD

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DHCR7 antibody (GTX130695) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DHCR7 antibody used in IHC (Paraffin sections) (IHC-P). GTX130695

GTX130695 IHC-P Image

DHCR7 antibody detects DHCR7 protein at cytoplasm in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
DHCR7 antibody (GTX130695) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DHCR7 antibody used in IHC (Paraffin sections) (IHC-P). GTX130695

GTX130695 IHC-P Image

DHCR7 antibody detects DHCR7 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
DHCR7 antibody (GTX130695) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DHCR7 antibody used in Western Blot (WB). GTX130695

GTX130695 WB Image

Orthogonal

MCF-7 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DHCR7 antibody (GTX130695) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Ohta-Shimizu M et al. Biol Pharm Bull 2021; 44 (4) : 485-493 New Inhibitory Effect of Latilactobacillus sakei UONUMA on the Cholesterol Biosynthesis Pathway in Human HepG2 Cells.
Hathazi D et al. Brain 2021; INPP5K and SIL1 associated pathologies with overlapping clinical phenotypes converge through dysregulation of PHGDH.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169