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DHFR antibody [AT5B2]

Anti-DHFR antibody [AT5B2] used in Western Blot (WB). GTX57632

Cat. No. GTX57632

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT5B2

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($399)
See all DHFR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT5B2 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human DHFR protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dihydrofolate reductase , DHFR , DHFRP1 , DYR , Dihydrofolate reductase

Background

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES

Anti-DHFR antibody [AT5B2] used in Western Blot (WB). GTX57632

GTX57632 WB Image

WB analysis of various samples using GTX57632 DHFR antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : 293T whole cell lysate
Loading : 40 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399