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DHFR antibody

KO/KD
Anti-DHFR antibody used in Immunoprecipitation (IP). GTX117705
Anti-DHFR antibody used in Western Blot (WB). GTX117705
Anti-DHFR antibody used in Western Blot (WB). GTX117705
Anti-DHFR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117705
Anti-DHFR antibody used in IHC (Paraffin sections) (IHC-P). GTX117705

Cat. No. GTX117705

Reference 2
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all DHFR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

PC-12

Predict Reactivity

Mouse, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DHFR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618840

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dihydrofolate reductase , DHFRP1 , DYR

Background

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DHFR antibody used in Immunoprecipitation (IP). GTX117705

GTX117705 IP Image

Immunoprecipitation of DHFR protein from 293T whole cell extracts using 5 μg of DHFR antibody (GTX117705).
Western blot analysis was performed using DHFR antibody (GTX117705).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-DHFR antibody used in Western Blot (WB). GTX117705

GTX117705 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with DHFR antibody (GTX117705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-DHFR antibody used in Western Blot (WB). GTX117705

GTX117705 WB Image

DHFR antibody detects DHFR protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
12% SDS-PAGE
DHFR antibody (GTX117705) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DHFR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117705

GTX117705 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using DHFR(GTX117705) antibody at 1:200 dilution.

Anti-DHFR antibody used in IHC (Paraffin sections) (IHC-P). GTX117705

GTX117705 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using DHFR(GTX117705) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Kuo WT et al. Oncol Rep 2017; (Epub) High * sensitivity of docetaxel-resistant A549 cells is mediated by TP53 status and downregulated thymidylate synthase.
Application : WB
Chiu LY et al. Oncogene 2016; (Epub) The ERK-ZEB1 pathway mediates epithelial-mesenchymal transition in * resistant lung cancer cells with suppression by vinca alkaloids.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

DHFR antibody Cat. No. GTX117705

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-DHFR antibody used in Western Blot (WB). GTX117705
Date : Anonymous submitted on 13-Aug-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 30 μg
Blocking : 5% skim milk, 24°C, 1Hr
Primary Antibody : 1:500, 24°C, 2Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169