APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa nuclear extract
Predict Reactivity
Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.35 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human DHX9. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886397
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DExH-box helicase 9 , DDX9 , LKP , NDH2 , NDHII , RHA
Cellular Localization
Cytoplasm , Nucleus , nucleolus
Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein with RNA helicase activity. It may participate in melting of DNA:RNA hybrids, such as those that occur during transcription, and may play a role in X-linked gene expression. It contains 2 copies of a double-stranded RNA-binding domain, a DEXH core domain and an RGG box. The RNA-binding domains and RGG box influence and regulate RNA helicase activity. [provided by RefSeq]
Database
DATA IMAGES
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GTX130971 IHC-P Image
DHX9 antibody detects DHX9 protein at cytoplasm and nucleus in CAL 27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded CAL 27 xenograft.
DHX9 antibody (GTX130971) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX130971 IHC-P Image
DHX9 antibody detects DHX9 protein at cytoplasm and nucleus in A549 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded A549 xenograft.
DHX9 antibody (GTX130971) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX130971 ICC/IF Image
DHX9 antibody detects DHX9 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DHX9 protein stained by DHX9 antibody (GTX130971) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX130971 WB Image
DHX9 antibody detects DHX9 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DHX9 antibody (GTX130971) diluted at 1:1000.
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REFERENCE
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REVIEW
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