DIP13B antibody [C2C3], C-term

Cat. No. GTX116299

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all DIP13B products

Datasheet
Document

View product citations for antibody GTX116299 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

NT2D1 , IMR32

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DIP13B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618707

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 , DIP13B

Cellular Localization

Early endosome membrane , Nucleus

Background

Required for the regulation of cell proliferation in response to extracellular signals mediated by an early endosomal compartment. Links Rab5 to nuclear signal transduction.

Database

Gene ID: 55198 APPL2
UniProt: Q8NEU8 APPL2

Research Area

DATA IMAGES

GTX116299 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: IMR32
7.5% SDS PAGE
GTX116299 diluted at 1:1000

GTX116299 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using DIP13B(GTX116299) antibody at 1:500 dilution.

GTX116299 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using DIP13B(GTX116299) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty