APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
U87-MG , SK-N-SH , IMR-32 , SK-N-AS
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human DIS3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885387
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease , 2810028N01Rik , EXOSC11 , KIAA1008 , RRP44 , dis3p
Cellular Localization
Cytoplasm , Nucleus , nucleolus
Background
Component of the exosome 3'->5' exoribonuclease complex. Required for the 3'-processing of the 7S pre-RNA to the mature nuclear complex. Also associated with the GTPase Ran. Has a 3'-5' exonuclease activity.
Database
Research Area
DATA IMAGES
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GTX115645 IHC-P Image
DIS3 antibody detects DIS3 protein at cytosol and nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
DIS3 antibody (GTX115645) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX115645 IHC-P Image
DIS3 antibody detects DIS3 protein at cytosol and nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
DIS3 antibody (GTX115645) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX115645 WB Image
DIS3 antibody detects DIS3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DIS3 antibody (GTX115645) diluted by 1:1000.
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GTX115645 ICC/IF Image
DIS3 antibody detects DIS3 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DIS3 protein stained by DIS3 antibody (GTX115645) diluted at 1:500.
Blue: Hoechst 33342 staining.
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REFERENCE
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REVIEW
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