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DLAT antibody

Anti-DLAT antibody used in IHC (Paraffin sections) (IHC-P). GTX109766
Anti-DLAT antibody used in Western Blot (WB). GTX109766
Anti-DLAT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109766

Cat. No. GTX109766

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DLAT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DLAT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176932

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dihydrolipoamide S-acetyltransferase , DLTA , E2 , PDC-E2 , PDCE2

Cellular Localization

Mitochondrion matrix

Background

The DLAT gene encodes dihydrolipoamide acetyltransferase (EC 2.3.1.12), the E2 subunit of the mammalian pyruvate dehydrogenase complex (PDC; EC 1.2.4.1) of the inner mitochondrial membrane. Patients with primary biliary cirrhosis (PBC; MIM 109720) show autoantibodies to DLAT.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-DLAT antibody used in IHC (Paraffin sections) (IHC-P). GTX109766

GTX109766 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma, using DLAT(GTX109766) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DLAT antibody used in Western Blot (WB). GTX109766

GTX109766 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
7.5% SDS PAGE
GTX109766 diluted at 1:10000

Anti-DLAT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109766

GTX109766 ICC/IF Image

DLAT antibody detects DLAT protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: DLAT protein stained by DLAT antibody (GTX109766) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Long DL et al. bioRxiv 2023; Glutathionylation of Pyruvate Dehydrogenase Complex E2 and Inflammatory Cytokine Production During Acute Inflammation Are Magnified By Mitochondrial Oxidative Stress.
DL Long et al. Redox Biol 2023; 65 : 102841 Glutathionylation of pyruvate dehydrogenase complex E2 and inflammatory cytokine production during acute inflammation are magnified by mitochondrial oxidative stress
Application : WB
Atsumi S et al. Commun Biol 2020; 3 (1) : 588 Focal adhesion ribonucleoprotein complex proteins are major humoral cancer antigens and targets in autoimmune diseases.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169