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DNAJB12 antibody [N2C3]

Anti-DNAJB12 antibody [N2C3] used in Western Blot (WB). GTX103639
Anti-DNAJB12 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX103639

Cat. No. GTX103639

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DNAJB12 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , MCF-7

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DNAJB12. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168492

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DnaJ heat shock protein family (Hsp40) member B12 , DJ10

Cellular Localization

Membrane

Background

DNAJB12 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-DNAJB12 antibody [N2C3] used in Western Blot (WB). GTX103639

GTX103639 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: H1299
C: HCT116
D: MCF-7
10% SDS PAGE
GTX103639 diluted at 1:1000

Anti-DNAJB12 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX103639

GTX103639 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using DNAJB12(GTX103639) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169